Our Lady of the Sign Church is an Eastern Orthodox church in the Žvėrynas district of Vilnius, built in 1903.
The idea of building a new Orthodox church in Vilnius came from Orthodox Brotherhood of the Holy Spirit, which also organised a collection of funds in the whole Russian Empire. The church, constructed in the most popular Neo-Byzantine style, was consecrated in 1903 by Iuvenaliy, the Orthodox archbishop of Vilnius. He also opened a school for poor children and a library which were to be run by the church's clergy. In order to commemorate the day, he granted to the newly established parish a copy of Our Lady of Kursk icon.
Unlike many other Orthodox churches in Vilnius, the church was not closed during World War I, nor during World War II. The Soviet government agreed to register it as a parish church in 1948.
Coordinates 54°41'26.895" N 25°15'26.26" E