Most long distance buses especially to Prague, Vienna or Bratislava stop here. Beware that this is not the main bus station in Brno.
Wikipedia (Czech) https://cs.wikipedia.org/wiki/Autobusov%25C3%25A9_n%25C3%25A1dra%25C5%25BE%25C3%25AD_Grand
Address Brno, Benešova tř.hotel GRAND, Benešova, Brno 60200, Czech Republic
Coordinates 49°11'36.322" N 16°36'51.995" E